Confocal Laser Scanning Microscopy (cLSM) allows 3-D visualization of metabolites, genes, proteins, or organisms using the auto-fluorescence of natural compounds like phenolic compounds or fluorescence properties of molecular probes linked to DNA, proteins or cellular structures.
cLSM has a long tradition at IMK-IFU. For our research we use this technique to study the in situ fluorescence of natural compounds involved in light attenuation, plant defense and plant microbe interactions. Applying FISH (fluorescent in situ hybridization) we follow occurrence and distribution of bacteria involved in N trace gas emissions on inner and outer plant surfaces. We also use this technique to analyze the regulation of the promoter region of the isoprene synthase (ISPS) gene in transgenic Arabidopsis and poplar plants transformed with promoter-EGFP (enhanced green fluorescent protein) fusions.
Contacts: & Hans Papen